There has been significant work done looking at using IMC to characterize the growth patterns of bacteria3-9 and even converting isothermal microcalorimetry data into biologically meaningful data such as growth rate, lag phase, or maximum growth.
This app note introduces the application of the measurement of metabolizable energy for growth of living organisms in food processing.
Thermogravimetric Analysis (TGA), which provides information about the thermal stability and decomposition rate of materials, is an excellent technique for rapidly comparing the behavior of materials tested with different flame retardants.
Thermogravimetric Analysis (TGA), which provides information about the thermal stability and decomposition rate of materials, is an excellent technique for rapidly comparing the behavior of materials tested with different flame retardants.
Adeno-associated viruses (AAVs) are a leading gene therapy platform but pose challenges regarding encapsidation efficiency and quality control. This work highlights the use of the Nano DSC to overcome some of the challenges associated with AAVs.
In this application note, we used the Nano DSC to monitor ST-DNA melting at a range of concentrations in phosphate buffer.
ITC has recently received increased attention from the field of material science, which necessitates the use of non-aqueous solvent systems. Herein, we elaborate on the development of a representative binding assay that measures the interactions of Ca2+ with a calcium ionophore in a methanol solvent system.
The interaction between the antigen binding site and an antigen is extremely specific, which has made antibodies very highly desirable reagents when developing a wide variety of antigen detection assays.
Isothermal titration calorimetry is a technique where heat is measured under constant pressure. This heat can be translated into enthalpy based on the First Law of Thermodynamics. Although the focus of the technique has been rooted in measuring biophysical phenomena in labs that specialized in calorimetry, it has seen growth as a general technique to quantitatively describe systems from proteins to nanomaterials.
Nano Differential Scanning Calorimetry (Nano DSC) and Isothermal Titration Calorimetry (ITC) are powerful label-free and immobilization free analytic tools for thermodynamic measurements of molecular binding, biomolecular thermostability, and enzyme kinetic reactions.
Nano Differential Scanning Calorimetry (Nano DSC) and Isothermal Titration Calorimetry (ITC) are powerful label-free and immobilization free analytic tools for thermodynamic measurements of molecular binding, biomolecular thermostability, and enzyme kinetic reactions.
Choosing to model DSC data can give additional insight into the unfolding process of a macromolecule or be helpful to deconvolute a multiple transition thermogram. This application note will discuss general guidelines of choosing the correct baseline and DSC model.