Biophysical Characterization of Antibody Drug Conjugates Using DSC
In this webinar, Dr. Colette Quinn discusses the use of differential scanning calorimetry (DSC) in protein stability characterization differences resulting from preparation changes of identical systems. Protein stability, particularly antibodies, can be determined via DSC without restrictions on buffers, excipients, and detergents. DSC, a native assay, works equally well for modified proteins such as antibody drug conjugates (ADC). This novel class of biotherapeutics can be characterized via calorimetry; in fact, a DSC thermogram is able to distinguish the different loads of the drug as well as the linker that was used in attaching the small organic molecule.