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The RH perfusion ampoule perfuses a gas of defined relative humidity over a sample and out through an exit tube. The relative humidity is controlled using two mass flow controllers, one of which passes gas directly to the sample and the other passes gas through two humidifying chambers prior to the sample. TAM Assistant™ software controls both the relative humidity and the total flow rate over the sample. The software is also used to change the humidity in a linear ramp or stepwise. The RH perfusion ampoule is available in 1, 4 and 20 ml versions. It is possible to use solvents other than water in the humidifying chambers which allows a gas of varying vapor pressure of a solvent to be passed over a sample.